Tests are categorised into 3 phases:
Phase 1: Mostly used in the development stages of products, either to prove an active substance within a product could be biocidal (for BPR submission to the list of approved active substances), or to prove that a co-formulant within a product has no biocidal activity – e.g., some essential oils have antimicrobial products, so if these are used to fragrance a product, they may wish to be screened to ensure that they are not contributing to reducing the number of organisms. In addition, sometimes several co-formulants mixed may react with one another to produce additional compounds that could also do the same thing.
Basic bactericidal activity of chemical disinfectants or antiseptics
Basic fungicidal / yeasticidal activity of chemical disinfectants or antiseptics
Basic sporicidal activity of chemical disinfectants or antiseptics
For these tests, NO interfering substance is used, and instead, water is used.
A standard contact time of 5 minutes (bacteria), 15 minutes (fungi/yeasts) and 30, 60 or 120 minutes (bacterial spores) is used for testing. For bacterial spores, results are compared to reference test solutions.
As Phase 1 testing does NOT take into consideration specific applications, therefore CANNOT be used to make a biocidal claim.
Phase 2: Phase 2 testing is broken down into 2 steps:
Step 1 (2/1) refers to quantitative suspension tests to establish that a product has biocidal activity (e.g., bactericidal, fungicidal, sporicidal, virucidal etc.) within a liquid suspension under simulated practical conditions appropriate for use.
Bactericidal / Food, industrial, domestic, and institutional area
Fungicidal / Food, industrial, domestic, and institutional area
Bactericidal / Medical area
Step 2 (2/2) refers to quantitative laboratory tests to establish biocidal activity (e.g., bactericidal, fungicidal, sporicidal, virucidal etc.) when applied to a surface or skin under simulated practical conditions.
Bactericidal & Fungicidal / Medical & Food, industrial, domestic, and institutional area
Bactericidal & Yeasticidal / Medical - Wipes
Bactericidal & Fungicidal / Medical (New 2021)
Phase 2, step 1, and phase 2, step 2 tests are generally needed in combination to support efficacy claims for chemical disinfectants or antiseptics. This is because dried-out (desiccated) microorganisms may be stressed and therefore offer different challenges to biocides, in comparison to those within a liquid suspension.
Phase 3: Phase 3 testing includes tests under actual conditions, rather than laboratory conditions (field tests or on-site tests). Guidelines are in place for the scope of the testing requirements; however no current standards are in use for phase 3 testing, but they may be developed in the future. For phase 3 testing, bespoke methods related to specific applications can be created by Melbec to aid product efficacy claims.
All biocidal testing can be carried out using standard organisms or can be adapted to include alternative organisms required to support individual product claims. If these standards are not appropriate to support a product claim, we have the technical knowledge and expertise to develop and validate a bespoke method relevant to your requirements.
To discover our full range of testing, please use the ‘Understanding Test Requirements’ page to discover which standard tests you may require to complete your biocidal product claim.