BS EN 1275:2005

BS EN 1275:2005 Fungicidal/Yeasticidal Activity
Mandatory test Organisms
C. albicans (Yeast)
A. brasiliensis (Mould)
Test temperature
Additional temperatures
20oC ± 1oC
4, 10 or 40oC ± 1oC
Contact Time
Additional contact times
15 mins ± 10 seconds
5 ± 5 seconds, 30 or 60 mins ± 10 seconds
Log Reduction
≥ 4 log

BS EN 1275:2005 Chemical disinfectants and antiseptics.

Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics. Test method and requirements (phase 1)

EN 1275 is a suspension test to determine whether a chemical disinfectant or antiseptic does or does not have a basic fungicidal or a basic yeasticidal activity.

Unlike Phase 2 testing, Phase 1 testing cannot be used for claim support for fungicidal or yeasticidal activity, as the testing does not include the use of interfering substance within the test and is therefore not as close to real life use of a product compared to Phase 2 testing. It can be used as an initial assessment for fungicidal/yeasticidal efficacy of products and may also be used to ensure that active substances chosen within product formulations are responsible for the activity of a product. It can also be used to assess co-formulants to ensure that they are not providing fungicidal or yeasticidal activity, particularly for those in combinations that may not be listed within UK or EU regulations for biocidal use.

The test parameters are simplified for this test, and therefore there are limitations to the microorganisms used, the test temperatures and the contact times used for testing.

For yeasticidal activity, only C. albicans is to be tested. For fungicidal activity, both C. albicans and A. brasiliensis are to be tested.

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