BS EN 14204:2012

EN 14204:2012
Obligatory Test Conditions
Additional Test Conditions
Test Organism
Mycobacterium avium
Any relevant test organism
Test Temperature
10oC ± 1oC
4oC ± 1oC
20oC ± 1oC
40oC ± 1oC
Contact Time
60 mins ± 10 secs
1 min
5 mins
10 mins
15 mins
30 mins
120 mins
(All ± 10 secs)
Interfering Substance
3 g/L bovine albumin
(low level soiling)
10 g/L yeast extract + 10 g/L bovine albumin
(high level soiling)
Any relevant substance
Log Reduction
≥ 4 log
≥ 4 log

BS EN 14204:2012 Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants and antiseptics used in the veterinary area – Test method and requirements (phase 2, step 1)

EN 14204:2012 is a suspension test for establishing whether a chemical disinfectant or antiseptic has mycobactericidal activity in the veterinary area.

Mycobacteria are often more resistant to chemical agents than vegetative bacteria, as they contain a waxy outer layer which protects them and makes them harder to kill. In addition, as the test is designed for the veterinary area, the standard test temperature is 10oC.

This laboratory test takes into account practical conditions of application of the product including contact time, temperature, test organisms and interfering substances, i.e. conditions which may influence its action in practical situations.

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